Colonel Carl Jenkins
A Very Smart Fellow
Steve (voice)
Neil Patrick Harris
Arnold Mosk
Lance
Dick Grayson / Nightwing (voice)
Patrick Winslow
Barry Allen / The Flash (voice)
Will Martin
Will Fratalli
Dr. Philip O'Hanlon (voice)
David Hart
Nathan Andrews
Jeff
Tobias
Travis Cleveland
Robert
The Shoe Fairy (uncredited) (archive footage)
Jim Stolpa
Two-Face / Black Manta (voice)
Danny Harland
Self
Foy
Desi Collings
Himself
Edward Broder
Jimmy Burden
Dr. Blowhole (voice)
Brian Hannigan
Narrator (voice)
Celebrity Dance-Off Host
Will Tweedy
Billy Johnson
David
Roger Martin
The Comic Shop Owner (voice)
Self - Actor
Steve Thompson
Jed (voice)
Doctor Horrible
Narrator
Doogie Howser M.D.
The Toymaker (archive footage)
Jeff Lonowski
Lou (voice)
Lonnie Tibbettes
Dan
Host / Himself
Self - Reader - Declaration of Independence
The Magic Mirror
Paul Keller
William Coit
Steve the Monkey (voice)
1st Lt. David Hettema / Sgt. William Manchester
Moyo (voice)
Self - Host
The Analyst
Host
Self - Performer
Screaming Reaver (uncredited)
Richard Fink
Adult Jake Doyle / Narrator
J.C. Leyendecker (voice)
Count Olaf
Todd Andrews
Tommy Remsen
Neil Patrick Harris (voice)
Self - Guest
Bryan Ryan
Henry McNeeley
Peter Corman
Replay / Johnny Morrow (voice)
Barney Stinson
Ray Thompson (voice)
Jonas
Doogie Howser
Joe Baxter
Max (voice)
Mike Hammond
Dr. Horrible
Bill
Lonnie
Prince Gumball
Howie Morrison
Barney Stinson (voice)
Himself - Host
Chester Creb
Himself - Guest Judge
Self - Judge
Neville LaRoy
Westley
(voice)
Michael Lawson
The Toymaker
The Dauphin
Peter Parker / Spider-Man (voice)
John Tagman
Philip (voice)
Self - Narrator (voice)
Buder
Guest
Music Meister (voice)
Henry Coltrane